Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd. Yang Nuryani • Otih Rostiana • Cheppy Syukur. Pengembangan Metode Fusi Protoplas dengan Tingkat Keberhasilan Pembentukan Fusan 25% untuk Memperoleh Tanaman Triploid Jeruk Garut Tanpa Biji. Request PDF on ResearchGate | Karakterisasi Dan Identifikasi Molekuler Fusan Hasil Fusi Protoplas Interspesies Chlorella pyrenoidosa dan.

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Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd – Neliti

Based on the distribution of phenols and lignin content. Phenol and lignin content in the somatic hybrids were dilferenl in Ihe respective pairs. Abstract Tangerine is a citrus seed Indonesia and including a true species of the genus Citrus.

The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Aceh patchouli Sidikalang and TT 75and the somatic hybrids.

The plant material used for the production of diploid callus is a population of diploid cells induced from embryonic nucellar tangerine. Jurnal Penelitian Tanaman Industri merupakan publikasi ilmiah primer yang memuat hasil penelitian Language Select Language English Indonesian. JavaScript is disabled for your browser. Ihe second group 9 clones contained high lignin. The best enzyme formulations for the isolation of protoplasts from diploid callus and leaves in vitro and protoplast Haploid pollen is macerozim 0.


Plants with triploid ploidy level usually have sterile fruit seedless. Abstract Evaluation of phenols and lignin in the root of hybrid patchouli Pogostemon cablin Benth.

First group consisted of somatic hybrids nos. Seedless citrus improvement can be done by manipulating the level ploidy. It has been found thai Ihe plain resistance lo nematode is closely related to phenols and lignin content of the root Therefore. Result showed that Java patchouli Girilaya contained phenols and lignin respectively View original from ejurnal. The genetic relationship among the somatic hybrids were estimated by using the index of similarity to perform genctical matrix and dendogram.

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Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd

DNA of the plant materials used were extracted by using the modified method of orozcocastulo et al. Evaluation of phenols and lignin in the root of hybrid patchouli Pogostemon cablin Benth.

Ihe level of resistance of different clones of somatic hybrids lo nematode was approached by evaluating ihe content of phenols and lignin of the roots The study was conducted al the laboratory of the Research Institute for food Crop Biotechnology, Bogor in November One of the problems faced in patchouli production is nematode infection Pratylenchus brachyurus. Improvement of the plant resistance to nematode in patchouli is dificult 10 be conducted through conventional method as the plant never llowcrs.



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Pusat Penelitian dan Pengembangan Perkebunan Jln. The second group was then classified into 2 minor groups. Article Tools Print this article.

The strategy chosen to assemble the triploid tangerine because the technique of protoplast isolation, fusion and regeneration of tangerine with diploid level has been mastered ploidy. Sidikalang and TT 75 were tested for their genetic variability and relationship. In the fusion of Girilaya x Sidikalang. Index of similarity among genotypes were calculated by using NTsys ver. Metadata Show full item record. Five random primers, OPD Haploid cell population repeatedly sub cultured every 4 weeks in a double protoplax media: Protopls cold treatment for more than 3 days on the response forming callus from pretreatment less than 3 days.

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